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  1. Laboratory Methods (Food)

Reagents Index for BAM

No.Reagent Name
R1Ammonium Acetate, 4 M
R2Ammonium Acetate, 0.25 M
R3Basic Fuchsin Staining Solution
R4Bicarbonate Buffer ( 0.1 M, pH 9.6)
R5Bovine Serum Albumin (BSA) (1 mg/ml)
R6Bovine Serum Albumin (1%) in Cholera Toxin ELISA Buffer
R7Bovine Serum Albumin (1% ) in PBS
R8Brilliant Green Dye Solution. 1%
R9Bromcresol Purple Dye Solution. 0.2%
R10Bromthymol Blue Indicator. 0.04%
R11Butterfield's Phosphate-Buffered Dilution Water
R12Catalase Test
R12aChlorine Solution, 200 ppm, Containing 0.1% Sodium Dodecyl Sulfate
R13Citric Acid (pH 4.0, 0.05 M)
R14Clark's Flagellar Stain
R15Coating Solution for V. vulnificus EIA
R16Crystal Violet Stain (for Bacteria)
R17Denhardt's Solution (50×)
R18Disinfectants (for preparation of canned foods for microbiological analysis)
R19Dulbecco's Phosphate-Buffered Saline (DPBS)
R20EDTA, 0.5 M
R21EIA (V. vulnificus) Wash Solution
R22ELISA Buffer (for Cholera Toxin Test)
R32aEndospore Stain (Schaeffer-Fulton)
R23Ethanol Solution, 70%
R24Evans Blue Dye Solution (commercially available)
R25Ferric Chloride, 10%
R26Removed from Bacteriological Analytical Manual. 1988.
R27Physiological Saline Solution
R28Gel Diffusion Agar, 1.2%
R29Gel-Phosphate Buffer
R30Giemsa Stain
R31Glycerin-Salt Solution (Buffered)
R98Glycine/NaCl Buffer, pH 7.6
R32Gram Stain (commercial staining solutions are satisfactory)
R33Hippurate Solution, 1%
R34Horseradish Peroxidase (color development solution)
R35Hybridization Mixture (6× SSC, 5× Denhardt's, 1 mM EDTA, pH 8.0)
R36Hydrochloric Acid, 1 N
R37Kinase Buffer (10×)
R38Kovacs' Reagent
R39Lithium Hydroxide, 0.1 N
R40Lugol's Iodine Solution
R94Lysis solution (0.5 M NaOH, 1.5M NaCl)
R41May-Grunwald Stain
R42McFarland Nephelometer
R43Removed from Bacteriological Analytical Manual. 1988.
R44Methyl Red Indicator
R45Methylene Blue Stain (Loeffler's)
R46Mineral Oil
R95Neutralizing solution (Maas II)
R47Ninhydrin Reagent (commercially available)
R48Nitrite Detection Reagents
R49North Aniline (Oil)-Methylene Blue Stain
R50Novobiocin Solution (100 mg/ml)
R51O/129 Disks (2,4-Diamino-6,7-diisopropyl pteridine)
R52O157 Monoclonal Antibody Solution
R53ONPG Test
R54Oxidase Reagent
R55Penicillinase (β-Lactamase)
R56Peptone Diluent, 0.1%
R97Peptone Diluent, 0.5%
R90Peptone-Tween-salt diluent (PTS)
R57Peroxidase Substrate for Membrane ELISA
R58Peroxidase Substrate Solution (ABTS)
(2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
R61Phosphate Saline Buffer (0.02 M, pH 7.3-7.4)
R62Phosphate Saline Solution (for Y-l LT Assay)
R60Phosphate-Buffered Saline (0.01 M , pH 7.5)
R59Phosphate-Buffered Saline (PBS), pH 7.4
R63Physiological Saline Solution 0.85% (Sterile)
R64Polymyxin B Disks, 50 Units
R65Potassium Hydroxide Solution. 40%
R66Saline Solution. 0.5% (Sterile)
R67Salts-Phosphate Buffered Saline Solution (Salts-PBS)
R9610% Sarkosyl Solution
R68Scintillation Fluid
R69Slide Preserving Solution
R70Sodium Bicarbonate Solution. 10%
R71Sodium Chloride Dilution Water, 2% and 3%
R72Sodium Chloride, 0.2 M Solution
R91Sodium desoxycholate-0.5% in sterile dH2O (String test)
R93(SSC/SDS) Sodium dodecyl sulfate
R92(SDS) Sodium dodecyl sulfate-10% in sterile dH2O
R73Sodium Hydroxide Solution (1 N)
R74Sodium Hydroxide Solution (10 N)
R75Sonicated Calf-Thymus or Salmon-Sperm DNA
R76Spicer-Edwards EN Complex Antibody Solution
R77Standard Saline Citrate (SSC) Solution (20%)
R78Tergitol Anionic 7
R79Thiazine Red R Stain (for S. aureus enterotoxin gel diffusion technique)
R80Tris ( 1.0 M, pH 8.0)
R84Tris-Buffered Saline (TABS), 1% or 3% Gelatin, or Tween 20
R81Tris-Buffered Saline (TBS) (PH 7.5)
R82Tris-Buffered Saline (TBS), with Gelatin
R83Tris-Buffered Saline (TBS)-Tween
R99Tris EDTA Primer TE, pH 8.0
R85Tris-EDTA-Triton X-100™(TET) Buffer
R86Triton X-100
R87Trypsin-EDTA Solution, lX
R88Verocytotoxin Antiserum
R89Voges-Proskauer (VP) Test Reagents