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  1. Laboratory Methods (Food)

Reagents Index for BAM

<< Bacteriological Analytical Manual (BAM) Main Page

No. Reagent Name
R1 Ammonium Acetate, 4 M
R2 Ammonium Acetate, 0.25 M
R3 Basic Fuchsin Staining Solution
R4 Bicarbonate Buffer ( 0.1 M, pH 9.6)
R5 Bovine Serum Albumin (BSA) (1 mg/ml)
R6 Bovine Serum Albumin (1%) in Cholera Toxin ELISA Buffer
R7 Bovine Serum Albumin (1% ) in PBS
R8 Brilliant Green Dye Solution. 1%
R9 Bromcresol Purple Dye Solution. 0.2%
R10 Bromthymol Blue Indicator. 0.04%
R11 Butterfield's Phosphate-Buffered Dilution Water
R12 Catalase Test
R12a Chlorine Solution, 200 ppm, Containing 0.1% Sodium Dodecyl Sulfate
R13 Citric Acid (pH 4.0, 0.05 M)
R14 Clark's Flagellar Stain
R15 Coating Solution for V. vulnificus EIA
R16 Crystal Violet Stain (for Bacteria)
R17 Denhardt's Solution (50×)
R18 Disinfectants (for preparation of canned foods for microbiological analysis)
R19 Dulbecco's Phosphate-Buffered Saline (DPBS)
R20 EDTA, 0.5 M
R21 EIA (V. vulnificus) Wash Solution
R22 ELISA Buffer (for Cholera Toxin Test)
R32a Endospore Stain (Schaeffer-Fulton)
R23 Ethanol Solution, 70%
R24 Evans Blue Dye Solution (commercially available)
R25 Ferric Chloride, 10%
R26 Removed from Bacteriological Analytical Manual. 1988.
R27 Physiological Saline Solution
R28 Gel Diffusion Agar, 1.2%
R29 Gel-Phosphate Buffer
R30 Giemsa Stain
R31 Glycerin-Salt Solution (Buffered)
R98 Glycine/NaCl Buffer, pH 7.6
R32 Gram Stain (commercial staining solutions are satisfactory)
R33 Hippurate Solution, 1%
R34 Horseradish Peroxidase (color development solution)
R35 Hybridization Mixture (6× SSC, 5× Denhardt's, 1 mM EDTA, pH 8.0)
R36 Hydrochloric Acid, 1 N
R37 Kinase Buffer (10×)
R38 Kovacs' Reagent
R39 Lithium Hydroxide, 0.1 N
R40 Lugol's Iodine Solution
R94 Lysis solution (0.5 M NaOH, 1.5M NaCl)
R41 May-Grunwald Stain
R42 McFarland Nephelometer
R43 Removed from Bacteriological Analytical Manual. 1988.
R44 Methyl Red Indicator
R45 Methylene Blue Stain (Loeffler's)
R46 Mineral Oil
R95 Neutralizing solution (Maas II)
R47 Ninhydrin Reagent (commercially available)
R48 Nitrite Detection Reagents
R49 North Aniline (Oil)-Methylene Blue Stain
R50 Novobiocin Solution (100 mg/ml)
R51 O/129 Disks (2,4-Diamino-6,7-diisopropyl pteridine)
R52 O157 Monoclonal Antibody Solution
R53 ONPG Test
R54 Oxidase Reagent
R55 Penicillinase (β-Lactamase)
R56 Peptone Diluent, 0.1%
R97 Peptone Diluent, 0.5%
R90 Peptone-Tween-salt diluent (PTS)
R57 Peroxidase Substrate for Membrane ELISA
R58 Peroxidase Substrate Solution (ABTS)
(2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
R61 Phosphate Saline Buffer (0.02 M, pH 7.3-7.4)
R62 Phosphate Saline Solution (for Y-l LT Assay)
R60 Phosphate-Buffered Saline (0.01 M , pH 7.5)
R59 Phosphate-Buffered Saline (PBS), pH 7.4
R63 Physiological Saline Solution 0.85% (Sterile)
R64 Polymyxin B Disks, 50 Units
R65 Potassium Hydroxide Solution. 40%
R66 Saline Solution. 0.5% (Sterile)
R67 Salts-Phosphate Buffered Saline Solution (Salts-PBS)
R96 10% Sarkosyl Solution
R68 Scintillation Fluid
R69 Slide Preserving Solution
R70 Sodium Bicarbonate Solution. 10%
R71 Sodium Chloride Dilution Water, 2% and 3%
R72 Sodium Chloride, 0.2 M Solution
R91 Sodium desoxycholate-0.5% in sterile dH2O (String test)
R93 (SSC/SDS) Sodium dodecyl sulfate
R92 (SDS) Sodium dodecyl sulfate-10% in sterile dH2O
R73 Sodium Hydroxide Solution (1 N)
R74 Sodium Hydroxide Solution (10 N)
R75 Sonicated Calf-Thymus or Salmon-Sperm DNA
R76 Spicer-Edwards EN Complex Antibody Solution
R77 Standard Saline Citrate (SSC) Solution (20%)
R78 Tergitol Anionic 7
R79 Thiazine Red R Stain (for S. aureus enterotoxin gel diffusion technique)
R80 Tris ( 1.0 M, pH 8.0)
R84 Tris-Buffered Saline (TABS), 1% or 3% Gelatin, or Tween 20
R81 Tris-Buffered Saline (TBS) (PH 7.5)
R82 Tris-Buffered Saline (TBS), with Gelatin
R83 Tris-Buffered Saline (TBS)-Tween
R99 Tris EDTA Primer TE, pH 8.0
R85 Tris-EDTA-Triton X-100™(TET) Buffer
R86 Triton X-100
R87 Trypsin-EDTA Solution, lX
R88 Verocytotoxin Antiserum
R89 Voges-Proskauer (VP) Test Reagents


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