BAM R45: Methylene Blue Stain (Loeffler's)

 

January 2001

Solution A

Methylene blue (90% dye content)	0.3 g
Ethanol (95%)	30

Solution B

Diluted potassium hydroxide (0.01%)	100 ml

Mix solutions A and B.

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Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.