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In this section: Laboratory Methods (Food)

BAM R60: 0.01 M Phosphate-Buffered Saline (pH 7.5)

January 2001

Stock solution (0.1 M)

Na₂HPO₄ (anhydrous)	12.0 g
NaH₂PO₄ · H₂0	2.2 g
NaCl	85.0 g
Distilled water	1 liter

Dissolve ingredients in distilled water and bring volume to 1 liter.

Dilute stock solution 1+9 in double distilled water. Mix well. Adjust pH to 7.5 with 0.1 N HCl or 0.1 N NaOH if necessary. Commercially available in dehydrated form from Difco Laboratories and BBL.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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