U.S. flag An official website of the United States government
  1. Home
  2. Food
  3. Science & Research (Food)
  4. Laboratory Methods (Food)
  5. BAM R31: Glycerin-Salt Solution (Buffered)
  1. Laboratory Methods (Food)

BAM R31: Glycerin-Salt Solution (Buffered)

 

January 2001

Glycerin (reagent grade) 100 ml
K2HPO4 (anhydrous) 12.4 g
KH2PO4 (anhydrous) 4 g
NaCl 4.2 g
Distilled water 900 ml

Distilled NaCl and bring volume to 900 ml with water. Add glycerin and phosphates. Adjust pH to 7.2. Autoclave 15 min at 121°C. For double strength (20%) glycerin solution, use 200 ml glycerin and 800 ml distilled water.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

 
Back to Top