BAM R38: Kovacs' Reagent

 

January 2001

p-Dimethylaminobenzaldehyde	5 g
Amyl alcohol (normal only)	75 ml
HCl (concentrated)	25 ml

Dissolve p-dimethylaminobenzaldehyde in normal amyl alcohol. Slowly add HCl. Store at 4°C. To test for indole, add 0.2-0.3 ml reagent to 5 ml of 24 h bacteria culture in tryptone broth. Dark red color in surface layer is positive test for indole.

---

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998