An official website of the United States government Here’s how you know

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

On Oct. 1, 2024, the FDA began implementing a reorganization impacting many parts of the agency. We are in the process of updating FDA.gov content to reflect these changes.

In this section: Laboratory Methods (Food)

BAM Media M72: Koser's Citrate Broth

Media Index for BAM

January 2001

NaNH₄HPO₄·4H₂O	1.5 g
KH₂PO₄ (monobasic)	1 g
MgSO₄·7H₂O	0.2 g
Sodium citrate·2H₂O	3 g
Distilled water	1 liter

Dispense into screw-cap tubes as desired. Autoclave 15 min at 121°C. Final pH, 6.7 ± 0.2. This formulation is listed in Official Methods of Analysis of AOAC International, and Standard Methods for the Examination of Wastewater of the APHA. It differs from the composition of commercially available dehydrated medium. The latter is satisfactory.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998

Top