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  1. Laboratory Methods (Food)

BAM Media M72: Koser's Citrate Broth

Media Index for BAM

January 2001

NaNH4HPO4·4H2O 1.5 g
KH2PO4 (monobasic) 1 g
MgSO4·7H2O 0.2 g
Sodium citrate·2H2O 3 g
Distilled water 1 liter

Dispense into screw-cap tubes as desired. Autoclave 15 min at 121°C. Final pH, 6.7 ± 0.2. This formulation is listed in Official Methods of Analysis of AOAC International, and Standard Methods for the Examination of Wastewater of the APHA. It differs from the composition of commercially available dehydrated medium. The latter is satisfactory.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998


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