U.S. flag An official website of the United States government
  1. Home
  2. Food
  3. Science & Research (Food)
  4. Laboratory Methods (Food)
  5. BAM Media M26: Bromcresol Purple Broth
  1. Laboratory Methods (Food)

BAM Media M26: Bromcresol Purple Broth

January 2001

Bacteriological Analytical Manual

Base

Peptone 10 g
Beef extract 3 g
NaCl 5 g
Bromcresol purple 0.04 g
Distilled water 1 liter

Dispense 2.5 ml portions of base solution into 13 × 100 mm test tubes containing inverted 6 × 50 mm fermentation tubes. Autoclave 10 min at 121°C. Final pH, 7.0 ± 0.2.

Sterilize stock solutions of carbohydrates (50% w/v) separately by autoclaving or, preferably, by filtration (0.2 µm pore size). Add 0.278 ± 0.002 ml stock carbohydrate solution to 2.5 ml basal medium to give 5% w/v final carbohydrate concentration.

For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.