Bacteriological Analytical Manual
|Beef extract||3 g|
|Bromcresol purple||0.04 g|
|Distilled water||1 liter|
Dispense 2.5 ml portions of base solution into 13 × 100 mm test tubes containing inverted 6 × 50 mm fermentation tubes. Autoclave 10 min at 121°C. Final pH, 7.0 ± 0.2.
Sterilize stock solutions of carbohydrates (50% w/v) separately by autoclaving or, preferably, by filtration (0.2 µm pore size). Add 0.278 ± 0.002 ml stock carbohydrate solution to 2.5 ml basal medium to give 5% w/v final carbohydrate concentration.
For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.