U.S. flag An official website of the United States government
  1. Home
  2. Food
  3. Science & Research (Food)
  4. Laboratory Methods (Food)
  5. BAM Media M158: Trypticase Soy-Polymyxin Broth
  1. Laboratory Methods (Food)

BAM Media M158: Trypticase Soy-Polymyxin Broth

January 2001

Prepare trypticase soy broth (M154) and dispense 15 ml portions into 20 × 150 mm tubes. Autoclave 15 min at 121°C. 

Polymyxin B solution, 0.15%

Dissolve 500,000 units polymyxin B sulfate in 33.3 ml distilled water. Filter-sterilize and store in the dark at 4°C until needed. Before use, add 0.1 ml sterile 0.15% polymyxin B solution to 15 ml tryptic soy broth, and mix thoroughly.

Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Back to Top