|Infusion broth||25 g|
|Distilled water||1 liter|
Heat with agitation to dissolve. Cool to 55°C and adjust pH to 7.4 ± 0.2. Dispense 4 ml portions into 13 × 100 mm screw-cap tubes. Autoclave 15 min at 121°C.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.