Drug-induced liver injury is the leading cause of acute liver failure in the United States causing significant morbidity and mortality in those that are affected. The assessment of endoplasmic reticulum (ER) stress levels is an important factor in monitoring potential liver toxicities and diseases.
FDA researchers have establishment two new cells lines (HepG2-Fluc-Gluc and HepG2-Fluc-Seap) to monitor ER stress levels quantitatively and efficiently, using Gaussia luciferase (Gluc) and secreted alkaline phosphatase (SEAP) as ER stress reporters. Validations studies have been completed using microarray and other biochemical and molecular analyses.
|Potential Commercial Applications||Competitive Advantages|
- Established cell lines, HepG2-Fluc-Gluc and HepG2-Fluc-Seap
- Lei Guo, Si Chen
- Chen, S. et. al. Sertraline induces endoplasmic reticulum stress in hepatic cells. Toxicology. 2014 Aug 1;322:78-88. PMID: 24865413
Product Area: in vitro system, cell line, research materials
FDA Reference No: E-2020-010
Ken Miliburne, J.D.
FDA Technology Transfer Program