An official website of the United States government Here’s how you know

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

In this section: Laboratory Methods (Food)

BAM Media M92: Malonate Broth

January 2001

Yeast extract	1 g
(NH₄)₂SO₄	2 g
K₂HPO₄	0.6 g
KH₂PO₄	0.4 g
NaCl	2 g
Sodium malonate	3 g
Glucose	0.25 g
Bromthymol blue	0.025 g
Distilled water	1 liter

Dissolve by heating, if necessary. Dispense 3 ml portions into 13 × 100 mm test tubes. Autoclave 15 min at 121°C. Final pH, 6.7 ± 0.2.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Top