BAM Media M91: MacConkey Agar
January 2001
| Proteose peptone or polypeptone |
3 g |
| Peptone or gelysate | 17 g |
| Lactose | 10 g |
| Bile salts No. 3 or bile salts mixture |
1.5 g |
| NaCl | 5 g |
| Neutral red | 0.03 g |
| Crystal violet | 0.001 g |
| Agar | 13.5 g |
| Distilled water | 1 liter |
Suspend ingredients and heat with agitation to dissolve. Boil 1-2 min. Autoclave 15 min at 121°C, cool to 45-50°C, and pour 20 ml portions into sterile 15 × 100 mm petri dishes. Dry at room temperature with lids closed.
DO NOT USE WET PLATES. Final pH, 7.1 ± 0.2.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.