BAM Media M39: Christensen Citrate Agar
January 2001
Bacteriological Analytical Manual
| Sodium citrate | 3 g |
| Glucose | 0.2 g |
| Yeast extract | 0.5 g |
| Cysteine monohydrochloride | 0.1 g |
| Ferric ammonium citrate | 0.4 g |
| KH2PO4 | 1 g |
| NaCl | 5 g |
| Sodium thiosulfate | 0.08 g |
| Phenol red | 0.012 g |
| Agar | 15 g |
| Distilled water | 1 liter |
Suspend ingredients, mix thoroughly and heat with occasional agitation. Boil about 1 min to dissolve ingredients. Fill 16 × 150 mm tubes 1/3 full and cap or plug to maintain aerobic conditions. Autoclave for 15 min at 121°C. Final pH, 6.9 ± 0.2. Before media solidify, incline tubes to obtain 4-5 cm slant and 2-3 cm butt. The Difco formulation does not include ferric ammonium citrate and sodium thiosulfate.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.