BAM Media M2: Acetamide Medium
January 2001
Stock basal medium
| KH2PO4 | 0.5 M | 14 ml |
|---|---|---|
| K2HPO4 | 0.5 M | 6 ml |
| Agar | 0.5 g | |
| Distilled water | 400 ml | |
Heat with agitation to dissolve agar. Add 1 ml PR-CV (500× concentrate).
Stock acetamide, 1%
| Acetamide | 1 g |
|---|---|
| Distilled water | 100 ml |
Store over CHCl3 in screw-cap container. Stable indefinitely at room temperature.
PR-CV (500× concentrate)
| Phenol red | 2 g |
|---|---|
| Crystal violet | 0.2 g |
| Distilled water | 200 ml |
Add 5 N NaOH until ingredients are dissolved.
Final medium
Add 0.8 ml basal medium to 13 × 100 mm tube. Add 0.2 ml acetamide solution. Steam (100°C) 10 min. Cool.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.