BAM Media M174: Violet Red Bile Agar (VRBA)
January 2001
| Yeast extract | 3.0 g |
| Peptone or gelysate | 7.0 g |
| NaCl | 5.0 g |
| Bile salts or bile salts No. 3 | 1.5 g |
| Lactose | 10.0 g |
| Neutral red | 0.03 g |
| Crystal violet | 0.002 g |
| Agar | 15.0 g |
| Distilled water | 1.0 liter |
Suspend ingredients in distilled water and let stand for a few min. Mix thoroughly and adjust to pH 7.4 ± 0.2. Heat with agitation and boil for 2 min. Do not sterilize. Before use, cool to 45°C and use as a plating medium. After solidification, add a cover layer above the agar of approximately 3.0 to 4.0 ml to prevent surface growth and spreading of colonies.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.