BAM Media M155: Trypticase (Tryptic) Soy Broth (TSB) with Glycerol
January 2001
| Trypticase peptone (tryptone) | 17 g |
| Phytone peptone (soytone) | 3 g |
| NaCl | 15 g |
| K2HPO4 | 2.5 g |
| Glycerol | 240 ml |
| Distilled water | 760 ml |
Suspend ingredients in distilled water and heat to dissolve. Dispense into bottles or vials. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.