BAM Media M148: Toluidine Blue-DNA Agar
January 2001
| Deoxyribonucleic acid (DNA) | 0.3 g |
|---|---|
| Agar | 10 g |
| CaCl2 (anhydrous) | 1.1 mg |
| NaCl | 10 g |
| Toluidine blue O | 0.083 g |
| Tris (hydroxymethyl) aminomethane | 6.1 g |
| Distilled water | 1 liter |
Dissolve Tris (hydroxymethyl) aminomethane in 1 liter distilled water. Adjust pH to 9.0. Add the remaining ingredients except toluidine blue O and heat to boiling to dissolve. Dissolve toluidine blue O in medium. Dispense to rubber-stoppered flasks. Sterilization is not necessary if used immediately. The sterile medium is stable at room temperature for 4 months and is satisfactory after several melting cycles.
| Storage | room temperature |
| Shelf life | 4 months |
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.