U.S. flag An official website of the United States government
  1. Home
  2. Food
  3. Science & Research (Food)
  4. Laboratory Methods (Food)
  5. BAM Media M102: Motility-Nitrate Medium, Buffered (for C. perfringens)
  1. Laboratory Methods (Food)

BAM Media M102: Motility-Nitrate Medium, Buffered (for C. perfringens)

January 2001

Beef extract 3 g
Peptone (Difco) 5 g
KNO3 1 g
Na2HPO3 2.5 g
Agar 3 g
Galactose 5 g
Glycerin (reagent grade) 5 ml
Distilled water 1 liter

Dissolve all ingredients except agar. Adjust pH to 7.3 ± 0.1. Add agar and heat to dissolve. Dispense 11 ml portions into 16 > 150 mm tubes. Autoclave 15 min at 121°C. If not used within 4 h, heat 10 min in boiling water or flowing steam. Chill in cold water.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

 
Back to Top