|Alcohol (absolute)||100 ml|
|Potassium hydroxide||40 g|
|Distilled water to make 100 ml|
Voges-Proskauer (VP) test
Transfer 1 ml of 48 h culture to test tube and add 0.6 ml solution 1 and 0.2 ml solution 2. Shake after adding each solution. To intensify and speed reaction, add a few creatine crystals to mixture. Let stand at room temperature. Read results 4 h after adding reagents.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.