|Trypsin (1:250)*||0.05 g|
|EDTA, disodium salt||0.02 g|
|NaCl, 0.9%||100 ml|
*1 g Trypsin (1:250) will digest 250 g casein substrate under standard conditions.
Dissolve trypsin and EDTA in the NaCl diluent. Filter-sterilize through 0.22 µm membrane.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.