BAM R87: Trypsin-EDTA Solution, 1X
January 2001
Trypsin (1:250)* | 0.05 g |
EDTA, disodium salt | 0.02 g |
NaCl, 0.9% | 100 ml |
*1 g Trypsin (1:250) will digest 250 g casein substrate under standard conditions.
Dissolve trypsin and EDTA in the NaCl diluent. Filter-sterilize through 0.22 µm membrane.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.