|Methanol (cold)||20 ml|
Dissolve 4-chloro-1-naphthol in cold methanol. Store at -20°C.
|Hydrogen peroxide, 30%||60 1|
|Tris-buffered saline (TBS)||100 ml|
Add 30% H2O2 to TBS at room temperature.
Immediately before use, mix ice cold Solution A with room temperature Solution B.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.