BAM R49: North Aniline (Oil) - Methylene Blue Stain
January 2001
| Aniline oil | 3 ml |
| Ethanol (95%) | 10 ml |
| HCl (concentrated) | 1.5 ml |
| Methylene blue (saturated solution) | 30 ml |
| Distilled water to make | 100 ml |
Mix aniline oil with ethanol. Slowly add HCl with constant agitation. Add saturated methylene blue solution, dilute to 100 ml with water, and filter.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.