|HRP color development reagent||60 mg|
|Ice cold methanol||20 ml|
Mix to dissolve. Protect from light and prepare fresh.
|Ice cold hydrogen peroxide, 30%||60 ml|
|Tris-buffered saline||100 ml|
Prepare fresh before use. Mix ice cold solution A with room temperature solution B. Use immediately.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.