|Glycerin (reagent grade)||100 ml|
|K2HPO4 (anhydrous)||12.4 g|
|KH2PO4 (anhydrous)||4 g|
|Distilled water||900 ml|
Distilled NaCl and bring volume to 900 ml with water. Add glycerin and phosphates. Adjust pH to 7.2. Autoclave 15 min at 121°C. For double strength (20%) glycerin solution, use 200 ml glycerin and 800 ml distilled water.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.