An official website of the United States government Here’s how you know

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

In this section: Laboratory Methods (Food)

BAM R28: Gel Diffusion Agar, 1.2%

January 2001

NaCl	8.5 g
Sodium barbital	8.0 g
Merthiolate (crystalline)	0.1 g
Noble special agar (Difco)	12.0 g
Distilled water	1 liter

Dissolve NaCl, sodium barbital, and merthiolate in 900 ml distilled water. Adjust pH to 7.4 with 1 N HCl and/or 1 N NaOH. Bring volume to l liter. Add Noble agar. Melt agar mixture in Arnold steamer. Filter in steamer, while hot, through 2 layers of analytical grade filter paper (e.g., No. 588, Schleicher and Schuell or equivalent). Dispense in small (15-25 ml) portions into 4 oz prescription bottles. Do not remelt more than twice.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Top