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In this section: Laboratory Methods (Food)

BAM Media M99: Motility-Indole-Ornithine (MIO) Medium

Media Index for BAM

January 2001

Yeast extract	3 g
Peptone	10 g
Tryptone	10 g
L-Ornithine HCl	5 g
Dextrose	1 g
Agar	2 g
Bromcresol purple	0.02 g
Distilled water	1 liter

Dispense 4 ml portions into 13 × 100 mm tubes. Autoclave 15 min at 121°C. Final pH, 6.5 ± 0.2.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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