Bacteriological Analytical Manual
|Peptone or gelysate||17 g|
|Bile salts No. 3
or bile salts mixture
|Neutral red||0.03 g|
|Crystal violet||0.001 g|
|Distilled water||1 liter|
Suspend ingredients and heat with agitation to dissolve. Boil 1-2 min. Autoclave 15 min at 121°C, cool to 45-50°C, and pour 20 ml portions into sterile 15 × 100 mm petri dishes. Dry at room temperature with lids closed.
DO NOT USE WET PLATES. Final pH, 7.1 ± 0.2.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.