BAM Media M89: Lysine Iron Agar (Edwards and Fife)
January 2001
| Gelysate or peptone | 5 g |
| Yeast extract | 3 g |
| Glucose | 1 g |
| L-Lysine hydrochloride | 10 g |
| Ferric ammonium citrate | 0.5 g |
| Sodium thiosulfate (anhydrous) | 0.04 g |
| Bromcresol purple | 0.02 g |
| Agar | 15 g |
| Distilled water | 1 liter |
Heat to dissolve ingredients. Dispense 4 ml portions into 13 × 100 mm screw-cap tubes. Autoclave 12 min at 121°C. Let solidify in slanted position to form 4 cm butts and 2.5 cm slants. Final pH, 6.7 ± 0.2.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.