An official website of the United States government Here’s how you know

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

On Oct. 1, 2024, the FDA began implementing a reorganization impacting many parts of the agency. We are in the process of updating FDA.gov content to reflect these changes.

In this section: Laboratory Methods (Food)

BAM Media M86: Lysine Arginine Iron Agar (LAIA)

Media Index for BAM

January 2001

Peptone	5 g
Yeast extract	3 g
Glucose	1 g
L-Lysine	10 g
L-Arginine	10 g
Ferric ammonium citrate	0.5 g
Sodium thiosulfate	0.04 g
Bromcresol purple	0.02 g
Agar	15 g

Adjust pH to 6.8. Heat to boiling and dispense 5 ml into each 13 × 100 mm screw-cap culture tube. Autoclave at 121°C for 12 min. Cool tubes in slanted position. (This medium may also be prepared by supplementing Difco lysine iron agar (LIA) with 10 g L-arginine per liter.)

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Top