|Yeast extract, 0.3%||3 g|
|Distilled water||1 liter|
Heat to dissolve ingredients. Dispense 4 ml portions to 13 × 100 mm screw-cap tubes. Autoclave 15 min at 121°C. Cool and tighten caps for storage. No pH adjustment is necessary.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.