BAM Media M81: Lithium Chloride-Phenylethanol-Moxalactam (LPM) Medium
|Phenylethanol agar (Difco)||35.5 g|
(NOTE: not glycine)
|Lithium chloride||5 g|
|Moxalactam stock solution,
1% in phosphate buffer, pH 6.0
|Distilled water||1 liter|
Sterilize medium (without moxalactam) at 121°C for 15 min. Cool to 48-50°C and add filter-sterilized moxalactam solution.
Moxalactam stock solution
Consists of 1 g moxalactam salt (ammonium or sodium) in 100 ml 0.1 M potassium phosphate buffer, pH 6.0. Store filter-sterilized stock solution frozen in 2 ml aliquots.
Moxalactam (Eli Lilly Co.) is retailed by Sigma Chemical Co. The LPM medium is most effective in the Henry illumination system when poured thin, i.e., 12-15 ml per standard petri dish. To avoid drying of thin agar, refrigerate and use plates rapidly. LPM basal medium is commercially available as a powder.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.