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In this section: Laboratory Methods (Food)

BAM Media M78: Letheen Agar (Modified)

Media Index for BAM

January 2001

Letheen agar (Difco or BBL)	32 g
Trypticase peptone	5 g
Thiotone peptone	10 g
Yeast extract	2 g
NaCl	5 g
Sodium bisulfite	0.1 g
Agar	5 g
Distilled water	1 liter

Heat with agitation to dissolve agar. Autoclave 15 min at 121°C. Aseptically dispense 20 ml into 15 × 100 mm petri dishes. Final pH, 7.2 ± 0.2.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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