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In this section: Laboratory Methods (Food)

BAM Media M77: Lauryl Tryptose MUG (LST-MUG) Broth

Media Index for BAM

January 2001

Tryptose or trypticase	20 g
Lactose	5 g
K₂HPO₄	2.75 g
KH₂PO₄	2.75 g
NaCl	5 g
Sodium lauryl sulfate	0.1 g
4-Methylumbelliferyl- β-D-glucuronide (MUG)*	50 mg
Distilled water	1 liter

Prepare lauryl tryptose broth and add MUG. Dissolve with gentle heat if necessary. Dispense 10 ml portions into 20 × 150 mm test tubes containing inverted 10 × 75 mm fermentation tubes. Autoclave 15 min at 121°C. Final pH, 6.8 ± 0.2.

*MUG may be obtained from several sources, e.g., HACH Co., Loveland, CO.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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