|Tryptose or trypticase||20 g|
|Sodium lauryl sulfate||0.1 g|
|Distilled water||1 liter|
Prepare lauryl tryptose broth and add MUG. Dissolve with gentle heat if necessary. Dispense 10 ml portions into 20 × 150 mm test tubes containing inverted 10 × 75 mm fermentation tubes. Autoclave 15 min at 121°C. Final pH, 6.8 ± 0.2.
*MUG may be obtained from several sources, e.g., HACH Co., Loveland, CO.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.