|Beef extract||3 g|
|Distilled water||1 liter|
Dispense 225 ml portions into 500 ml Erlenmeyer flasks. After autoclaving 15 min at 121°C and just before use, aseptically adjust volume to 225 ml. Final pH, 6.9 ± 0.2.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.