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In this section: Laboratory Methods (Food)

BAM Media M73: L-15 Medium (Modified) Leibovitz

Media Index for BAM

January 2001

D-Galactose	90 mg	L-Tyrosine	300 mg
Phenol red, Na	10 mg	DL-Valine	200 mg
Sodium pyruvate	550 mg	D-Calcium pantothenate	1 mg
DL--Alanine	450 mg	Choline chloride	1 mg
L-Arginine (free base)	500 mg	Folic acid	1 mg
L-Asparagine·H₂O	250 mg	i-Inositol	2 mg
L-Cysteine (free base)	120 mg	Nicotinamide	1 mg
L-Glutamine	300 mg	Pyridoxine HCl	1 mg
Glycine	200 mg	Riboflavin-5-phosphate, Na	0.1 mg
L-Histidine (free base)	250 mg	Thiamin monophosphate·2H₂O	1.0 mg
L-Isoleucine	250 mg	CaCl₂ (anhydrous)	140 mg
L-Leucine HC1	125 mg	KC1	400 mg
DL-Methionine	150 mg	KH₂PO₄	60 mg
L-Phenylalanine	250 mg	MgCl₂ (anhydrous)	93.68 mg
L-Serine	200 mg	NaCl	8000 mg
DL-Threonine	600 mg	Na₂HPO₄ (anhydrous)	190 mg
L-Tryptophan	20 mg	Distilled water	1 liter

Filter through 0.20 µm membrane and dispense into 2 liter Erlenmeyer flask. Final pH, 7.5. Cap and store at 5°C.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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