BAM Media M71: Kligler Iron Agar
|Polypeptone peptone||20 g|
|Ferric ammonium citrate||0.5 g|
|Sodium thiosulfate||0.5 g|
|Phenol red||0.025 g|
|Distilled water||1 liter|
Heat with agitation to dissolve. Dispense into 13 × 100 mm screw-cap tubes and autoclave 15 min at 121°C. Cool and slant to form deep butts. Final pH, 7.4 ± 0.2.
Commercially available from Difco, BBL, and Oxoid.
For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.