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  1. Laboratory Methods (Food)

BAM Media M71: Kligler Iron Agar

January 2001

Bacteriological Analytical Manual

Polypeptone peptone 20 g
Lactose 20 g
Dextrose 1 g
NaCl 5 g
Ferric ammonium citrate 0.5 g
Sodium thiosulfate 0.5 g
Agar 15 g
Phenol red 0.025 g
Distilled water 1 liter

Heat with agitation to dissolve. Dispense into 13 × 100 mm screw-cap tubes and autoclave 15 min at 121°C. Cool and slant to form deep butts. Final pH, 7.4 ± 0.2.

Commercially available from Difco, BBL, and Oxoid.

For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.