|Trypticase (or casitone)||2 g|
|Phenol red, 1.5% solution||2 ml|
|Distilled water||1 liter|
Heat with agitation to dissolve agar. Adjust pH to 7.3 ± 0.2. Dispense 100 ml portions to flasks. Autoclave 15 min at 121°C. Cool to 50°C.
Dissolve 10 g quantities of carbohydrates in 100 ml distilled water. Sterilize by filtration through 0.22 µm membrane. Add 10 ml concentrate to 90 ml melted base and mix. Aseptically dispense 3 ml portions to sterile 13 × 100 mm tubes.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.