An official website of the United States government Here’s how you know

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

On Oct. 1, 2024, the FDA began implementing a reorganization impacting many parts of the agency. We are in the process of updating FDA.gov content to reflect these changes.

In this section: Laboratory Methods (Food)

BAM Media M7: AKI Medium

Media Index for BAM

January 2001

Peptone	15 g
Yeast extract	4 g
NaCl	5 g
Distilled water	970 ml
NaHCO₃, 10% aqueous, filter-sterilized	30 ml

On day of use, dissolve peptone, yeast extract, and NaCl in distilled water. Autoclave 15 min at 121°C. Cool. Add 30 ml freshly prepared, filter-sterilized NaHCO₃, and mix. Dispense aseptically into screw-capped tubes (use 15 ml for 16 × 125 mm tubes). Final pH, 7.4 ± 0.2.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Top