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  1. Laboratory Methods (Food)

BAM Media M7: AKI Medium

Media Index for BAM

January 2001

Peptone 15 g
Yeast extract 4 g
NaCl 5 g
Distilled water 970 ml
10% aqueous, filter-sterilized
30 ml

On day of use, dissolve peptone, yeast extract, and NaCl in distilled water. Autoclave 15 min at 121°C. Cool. Add 30 ml freshly prepared, filter-sterilized NaHCO3, and mix. Dispense aseptically into screw-capped tubes (use 15 ml for 16 × 125 mm tubes). Final pH, 7.4 ± 0.2.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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