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In this section: Laboratory Methods (Food)

BAM Media M69: King's B Medium

Media Index for BAM

January 2001

Proteose peptone No. 3	20 g
Glycerol, C.P.	10 ml
K₂HPO₄	1.5 g
MgSO₄	1.5 g
Agar	15 g
Distilled water	1 liter

Add all ingredients except MgSO₄. Heat with agitation to dissolve agar. Adjust pH to 7.2 ± 0.2. Add MgSO₄ slowly and mix. Dispense 4 ml portions to 13 × 100 tubes. Autoclave 15 min at 121°C.

Incline tubes to give half butt and half slant.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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