|Proteose peptone No. 3||20 g|
|Glycerol, C.P.||10 ml|
|Distilled water||1 liter|
Add all ingredients except MgSO4. Heat with agitation to dissolve agar. Adjust pH to 7.2 ± 0.2. Add MgSO4 slowly and mix. Dispense 4 ml portions to 13 × 100 tubes. Autoclave 15 min at 121°C.
Incline tubes to give half butt and half slant.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.