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In this section: Laboratory Methods (Food)

BAM Media M63: Hugh-Leifson Glucose Broth (HLGB)

Media Index for BAM

January 2001

Peptone	2 g
Yeast extract	0.5 g
NaCl	30 g
Dextrose	10 g
Bromcresol purple	0.015 g
Agar	3 g
Distilled water	1 liter

Heat with agitation to dissolve agar. Adjust pH to 7.4 ± 0.2. Autoclave 15 min at 121°C.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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