An official website of the United States government Here’s how you know

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

On Oct. 1, 2024, the FDA began implementing a reorganization impacting many parts of the agency. We are in the process of updating FDA.gov content to reflect these changes.

In this section: Laboratory Methods (Food)

BAM Media M61: Hektoen Enteric (HE) Agar

Media Index for BAM

January 2001; updated August 2010

Storage: May be stored up to 30 days under refrigeration (4 ± 2°C).

Peptone	12 g	NaCl	5 g
Yeast extract	3 g	Sodium thiosulfate	5 g
Bile salts No. 3	9 g	Ferric ammonium citrate	1.5 g
Lactose	12 g	Bromthymol blue	0.065 g
Sucrose	12 g	Acid fuchsin	0.1 g
Salicin	2 g	Agar	14.0 g
Distilled water			1 liter

Heat to boiling with frequent agitation to dissolve. Boil no longer than 1 min. Do not overheat. Cool in water bath. Pour 20 ml portions into sterile 15 × 100 mm petri dishes. Let dry 2 h with lids partially removed. Final pH, 7.5 ± 0.2.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Top