|Beef heart infusion (Difco)||500 g|
|Distilled water||1 liter|
|Blood Agar Base No. 2 (Difco)||1 liter|
|Proteose peptone||15 g|
|Liver digest||2.5 g|
|Yeast extract||5 g|
Autoclave either base at 121°C for 15 min. If preparing blood agar, reduce water to 950 ml. Add 50 ml defibrinated (whole or lysed) horse blood and FBP (4 ml to agar + blood) after autoclaving and cooling to 48°C. Final pH, 7.4 ± 0.2.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.