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  1. Laboratory Methods (Food)

BAM Media M53: Esculin Agar, Modified (CDC)

January 2001

Bacteriological Analytical Manual

Infusion agar 40 g
Esculin 1 g
Ferric citrate 0.5 g
Distilled water 1 liter

Heat with agitation to dissolve agar. Cool to 55°C. Adjust pH to 7.0 ± 0.2. Dispense 4 ml portions to 13 × 100 mm tubes. Autoclave 15 min at 121°C. Slant tubes.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.