BAM Media M53: Esculin Agar, Modified (CDC)
January 2001
Infusion agar | 40 g |
Esculin | 1 g |
Ferric citrate | 0.5 g |
Distilled water | 1 liter |
Heat with agitation to dissolve agar. Cool to 55°C. Adjust pH to 7.0 ± 0.2. Dispense 4 ml portions to 13 × 100 mm tubes. Autoclave 15 min at 121°C. Slant tubes.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.