U.S. flag An official website of the United States government

On Oct. 1, 2024, the FDA began implementing a reorganization impacting many parts of the agency. We are in the process of updating FDA.gov content to reflect these changes.

U.S. Food and Drug Administration
  1. Home
  2. Food
  3. Science & Research (Food)
  4. Laboratory Methods (Food)
  5. BAM Media M5: AE Sporulation Medium, Modified (for C.perfringens)
  1. Laboratory Methods (Food)

BAM Media M5: AE Sporulation Medium, Modified (for C.perfringens)

Media Index for BAM

January 2001

 
Polypeptone 10.0 g
Yeast extract 10.0 g
Na2HPO4 4.36 g
KH2PO4 0.25 g
Ammonium acetate 1.5 g
MgSO4·7H2O 0.2 g
Distilled water 1 liter

Dissolve ingredients and adjust to pH 7.5 ± 0.1, using 2 M sodium carbonate. Dispense 15 ml into 20 × 150-mm screw-cap tubes and sterilize by autoclaving for 15 min at 121°C.

After sterilization, add 0.6 ml of sterilized 10% raffinose and 0.2 ml each of filter-sterilized 0.66 M sodium carbonate and 0.32% cobalt chloride (CoCl2·6H2O) dropwise to each tube.

Check pH of one or two tubes; it should be 7.8 ± 0.1. Just before use, steam medium for 10 min; after cooling, add 0.2 ml of filter-sterilized 1.5% sodium ascorbate (prepared daily) to each tube.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Back to Top