BAM Media M46: (with Earle's salts and nonessential amino acids)

Media Index for BAM

January 2001

L-Alanine	8.9 mg	L-Valine	46.0 mg
L-Arginine HCl	126.0 mg	L-Tyrosine (disodium salt)	52.1 mg
L-Asparagine·H2O	150.0 mg	D-Calcium pantothenate	1.0 mg
L-Aspartic acid	13.3 mg	Choline chloride	1.0 mg
L-Cystine·2HCl	31.29 mg	Folic acid	1.0 mg
L-Glutamic acid	14.7 mg	Isoinositol	2.0 mg
L-Glutamine	292.0 mg	Nicotinamide	1.0 mg
L-Glycine	7.5 mg	Pyridoxal HCl	1.0 mg
L-Histidine HCl·H2O	42.0 mg	Riboflavin	0.1 mg
L-Isoleucine	52.0 mg	Thiamine HCl	1.0 mg
L-Leucine	52.0 mg	Glucose	1000.0 mg
L-Lysine HCl	72.5 mg	CaCl2·2H2O	265.0 mg
L-Methionine	15.0 mg	KCl	400.0 mg
D-Phenylalanine	32.0 mg	MgSO4·7H2O	200.0 mg
L-Proline	11.5 mg	NaCl	6800.0 mg
L-Serine	10.5 mg	NaHCO3	2200.0 mg
L-Threonine	48.0 mg	NaH2PO4·H2O	140.0 mg
L-Tryptophan	10.0 mg	Phenol red	10.0 mg
Distilled water			1 liter

Dissolve ingredients in distilled water. Sterilize by filtration. Final pH, 7.2 ± 0.2.

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Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.