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In this section: Laboratory Methods (Food)

BAM Media M45: Duncan-Strong (DS) Sporulation Medium, Modified (for C. perfringens)

Media Index for BAM

January 2001

Proteose peptone	15 g
Yeast extract	4 g
Sodium thioglycollate	1 g
Na₂HPO₄^.7H₂O	10 g
Raffinose	4 g
Distilled water	1 liter

Dissolve ingredients and sterilize by autoclaving for 15 min at 121°C. Adjust to pH 7.8 ± 0.1, using filter-sterilized 0.66 M sodium carbonate.

Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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