BAM Media M44: Decarboxylase Basal Medium (Arginine, Lysine, Ornithine)
|Peptone or gelysate||5 g|
|Yeast extract||3 g|
|Bromcresol purple||0.02 g|
|Distilled water||1 liter|
Adjust pH so that value after sterilization is 6.5 ± 0.2. Dispense 5 ml portions into 16 × 125 mm screw-cap tubes. Autoclave loosely capped tubes 10 min at 121°C. Screw the caps on tightly for storage and after inoculation. For control, use unsupplemented base.
|For||to 1 liter base, add:|
|Arginine Broth||5 g L-arginine|
|Lysine Broth (Falkow)||5 g L-lysine|
|Ornithine Broth||5 g L-ornithine|
For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.