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  1. Laboratory Methods (Food)

BAM Media M44: Decarboxylase Basal Medium (Arginine, Lysine, Ornithine)

January 2001

Bacteriological Analytical Manual

Base

Peptone or gelysate 5 g
Yeast extract 3 g
Glucose 1 g
Bromcresol purple 0.02 g
Distilled water 1 liter

Adjust pH so that value after sterilization is 6.5 ± 0.2. Dispense 5 ml portions into 16 × 125 mm screw-cap tubes. Autoclave loosely capped tubes 10 min at 121°C. Screw the caps on tightly for storage and after inoculation. For control, use unsupplemented base.

For to 1 liter base, add:
Arginine Broth 5 g  L-arginine
Lysine Broth (Falkow) 5 g L-lysine
Ornithine Broth 5 g L-ornithine

For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.


Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.