(a) Cooked meat medium
commercially available in dehydrated form
|Beef heart||454 g|
|Proteose peptone||20 g|
(not available commercially)
|Sodium thioglycollate||1 g|
|Soluble starch||1 g|
|Neutral red (1% aqueous)||5 ml|
|Distilled water||1 liter|
Adjust pH to 6.8 ± 0.2. Add 1 g dehydrated (a) and 15 ml (b) to 20 × 150 mm tubes. Let meat particles rehydrate. Autoclave 15 min at 121°C.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.