|Beef heart||454 g|
|Proteose peptone||20 g|
|Distilled water||1 liter|
Follow directions as in M38, or suspend 12.5 g commercial dehydrated cooked medium in 100 ml cold distilled water. Mix and let stand 15 min to wet particles thoroughly. Or distribute 1.25 g into 20 × 150 mm test tubes, add 10 ml cold distilled water, and mix thoroughly to wet all particles. Autoclave 15 min at 121°C. Final pH, 7.2 ± 0.2. Steam the sterilized medium and cool, without agitation, just before use.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.