Bacteriological Analytical Manual
|Sodium citrate||3 g|
|Yeast extract||0.5 g|
|Cysteine monohydrochloride||0.1 g|
|Ferric ammonium citrate||0.4 g|
|Sodium thiosulfate||0.08 g|
|Phenol red||0.012 g|
|Distilled water||1 liter|
Suspend ingredients, mix thoroughly and heat with occasional agitation. Boil about 1 min to dissolve ingredients. Fill 16 × 150 mm tubes 1/3 full and cap or plug to maintain aerobic conditions. Autoclave for 15 min at 121°C. Final pH, 6.9 ± 0.2. Before media solidify, incline tubes to obtain 4-5 cm slant and 2-3 cm butt. The Difco formulation does not include ferric ammonium citrate and sodium thiosulfate.
Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.